Characterization of the Filamentous Hemagglutinin-Like Protein FhaS in Bordetella bronchiseptica
نویسندگان
چکیده
منابع مشابه
Characterization of the filamentous hemagglutinin-like protein FhaS in Bordetella bronchiseptica.
Filamentous hemagglutinin (FHA) is a large (>200 kDa), rod-shaped protein expressed by bordetellae that is both surface-associated and secreted. FHA mediates bacterial adherence to epithelial cells and macrophages in vitro and is absolutely required for tracheal colonization in vivo. The recently sequenced Bordetella bronchiseptica genome revealed the presence of a gene, fhaS, that is nearly id...
متن کاملProduction of biomass and filamentous hemagglutinin by Bordetella bronchiseptica.
The mammalian pathogen Bordetella bronchiseptica was grown under controlled batch conditions with glutamate as the primary carbon and nitrogen source. First, a Box-Behnken statistical design quantified the effect of Mg, sulfate, and nicotinate on the antigen filamentous hemagglutinin (FHA) formation. Using lactic acid as a secondary carbon source for pH control, Mg, and SO₄ each negatively affe...
متن کاملCharacterization of BrkA expression in Bordetella bronchiseptica.
BrkA confers resistance to killing by complement in Bordetella pertussis. Complement resistance in Bordetella bronchiseptica was examined. Four B. bronchiseptica strains possessed the brkA gene; however, only three expressed the protein. Only the strain lacking BrkA was susceptible to complement. Introduction of the B. pertussis brkA gene restored BrkA expression to this strain but did not conf...
متن کاملMolecular characterization of Bordetella bronchiseptica filamentous haemagglutinin and its secretion machinery.
Two closely related pathogens, Bordetella pertussis and Bordetella bronchiseptica, share a number of virulence factors. Filamentous haemagglutinin (FHA) is widely regarded as the dominant adhesin of B. pertussis, and its multiple binding activities have been well characterized. This large protein is produced and secreted at high levels by B. pertussis and significantly lower levels by B. bronch...
متن کاملexpression, purification and characterization of three overlapping immunodominant recombinant fragments from bordetella pertussis filamentous hemagglutinin
background: filamentous hemagglutinin (fha) is one of the most important immunoprotective antigens of bordetella pertussis (b.pertussis) and a major component of the acellular pertussis vaccine. in the present study, three overlapping recombinant fragments from the immunodominant region of fha were produced and their immunogenicity was investigated. methods: three overlapping coding sequences o...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 2005
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.73.8.4960-4971.2005